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constitutive tdh3 promoter  (Addgene inc)


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    Structured Review

    Addgene inc constitutive tdh3 promoter
    Constitutive Tdh3 Promoter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/constitutive+tdh3+promoter/p2xFLAGhYAP1-WW+mutant+(Plasmid+%2317792)/pmc12491163-228-24-30
    Average 93 stars, based on 6 article reviews
    constitutive tdh3 promoter - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Protocol for inducible expression of small non-coding RNAs using a tet-on system in Saccharomyces cerevisiae
    Article Snippet: .. Note: This plasmid contains the reverse tetracycline transactivator (rtTA) sequence, modified to have high-sensitivity and low leakiness (rtTA-M2-SE-G72P), under the control of the strong, constitutive TDH3 promoter (originally obtained from Addgene Plasmid # 177924). ..


    Article Title: Protocol for inducible expression of small non-coding RNAs using a tet-on system in Saccharomyces cerevisiae
    Article Snippet: .. Note: This plasmid contains the reverse tetracycline transactivator (rtTA) sequence, modified to have high-sensitivity and low leakiness (rtTA-M2-SE-G72P), under the control of the strong, constitutive TDH3 promoter (originally obtained from Addgene Plasmid # 177924).3 This plasmid also contains the T7 polymerase gene, modified with a SV40 nuclear localization signal (NLS) between amino acids 10 and 11 and to have reduced abortive transcription (P266L; analogous to P278L in the T7 polymerase containing the NLS; originally obtained from the Alper lab4). ..

    Sequencing:

    Article Title: Protocol for inducible expression of small non-coding RNAs using a tet-on system in Saccharomyces cerevisiae
    Article Snippet: .. Note: This plasmid contains the reverse tetracycline transactivator (rtTA) sequence, modified to have high-sensitivity and low leakiness (rtTA-M2-SE-G72P), under the control of the strong, constitutive TDH3 promoter (originally obtained from Addgene Plasmid # 177924). ..

    Article Title: Protocol for inducible expression of small non-coding RNAs using a tet-on system in Saccharomyces cerevisiae
    Article Snippet: .. Note: This plasmid contains the reverse tetracycline transactivator (rtTA) sequence, modified to have high-sensitivity and low leakiness (rtTA-M2-SE-G72P), under the control of the strong, constitutive TDH3 promoter (originally obtained from Addgene Plasmid # 177924).3 This plasmid also contains the T7 polymerase gene, modified with a SV40 nuclear localization signal (NLS) between amino acids 10 and 11 and to have reduced abortive transcription (P266L; analogous to P278L in the T7 polymerase containing the NLS; originally obtained from the Alper lab4). ..

    Modification:

    Article Title: Protocol for inducible expression of small non-coding RNAs using a tet-on system in Saccharomyces cerevisiae
    Article Snippet: .. Note: This plasmid contains the reverse tetracycline transactivator (rtTA) sequence, modified to have high-sensitivity and low leakiness (rtTA-M2-SE-G72P), under the control of the strong, constitutive TDH3 promoter (originally obtained from Addgene Plasmid # 177924). ..


    Article Title: Protocol for inducible expression of small non-coding RNAs using a tet-on system in Saccharomyces cerevisiae
    Article Snippet: .. Note: This plasmid contains the reverse tetracycline transactivator (rtTA) sequence, modified to have high-sensitivity and low leakiness (rtTA-M2-SE-G72P), under the control of the strong, constitutive TDH3 promoter (originally obtained from Addgene Plasmid # 177924).3 This plasmid also contains the T7 polymerase gene, modified with a SV40 nuclear localization signal (NLS) between amino acids 10 and 11 and to have reduced abortive transcription (P266L; analogous to P278L in the T7 polymerase containing the NLS; originally obtained from the Alper lab4). ..

    Control:

    Article Title: Protocol for inducible expression of small non-coding RNAs using a tet-on system in Saccharomyces cerevisiae
    Article Snippet: .. Note: This plasmid contains the reverse tetracycline transactivator (rtTA) sequence, modified to have high-sensitivity and low leakiness (rtTA-M2-SE-G72P), under the control of the strong, constitutive TDH3 promoter (originally obtained from Addgene Plasmid # 177924). ..


    Article Title: Protocol for inducible expression of small non-coding RNAs using a tet-on system in Saccharomyces cerevisiae
    Article Snippet: .. Note: This plasmid contains the reverse tetracycline transactivator (rtTA) sequence, modified to have high-sensitivity and low leakiness (rtTA-M2-SE-G72P), under the control of the strong, constitutive TDH3 promoter (originally obtained from Addgene Plasmid # 177924).3 This plasmid also contains the T7 polymerase gene, modified with a SV40 nuclear localization signal (NLS) between amino acids 10 and 11 and to have reduced abortive transcription (P266L; analogous to P278L in the T7 polymerase containing the NLS; originally obtained from the Alper lab4). ..

    Transformation Assay:


    Expressing:


    Abortive Initiation Assay:


    Article Title: Protocol for inducible expression of small non-coding RNAs using a tet-on system in Saccharomyces cerevisiae
    Article Snippet: .. Note: This plasmid contains the reverse tetracycline transactivator (rtTA) sequence, modified to have high-sensitivity and low leakiness (rtTA-M2-SE-G72P), under the control of the strong, constitutive TDH3 promoter (originally obtained from Addgene Plasmid # 177924).3 This plasmid also contains the T7 polymerase gene, modified with a SV40 nuclear localization signal (NLS) between amino acids 10 and 11 and to have reduced abortive transcription (P266L; analogous to P278L in the T7 polymerase containing the NLS; originally obtained from the Alper lab4). ..



    Similar Products

    93
    Addgene inc constitutive tdh3 promoter
    Constitutive Tdh3 Promoter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/constitutive+tdh3+promoter/p2xFLAGhYAP1-WW+mutant+(Plasmid+%2317792)/pmc12491163-228-24-30
    Average 93 stars, based on 1 article reviews
    constitutive tdh3 promoter - by Bioz Stars, 2026-09
    93/100 stars
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